Anti-PKA C alpha Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	5566
Protein Name / Synonyms	PKA C alpha
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

MGC102831 antibody, MGC48865 antibody, PKACA antibody, PRKACA antibody, protein kinase A catalytic subunit antibody, "cAMP-dependent protein kinase catalytic subunit alpha, isoform 1 antibody", cAMP-dependent protein kinase catalytic subunit alpha antibody, PKA C-alpha antibody, "protein kinase, cAMP-dependent, catalytic, alpha antibody"

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

41 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 351 of PKA C alpha (Uniprot ID# P17612)

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: C2C12
10% SDS PAGE
107-10691 diluted at 1:1000

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PKA alpha(107-10691) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.

Sample(30 µg of whole cell lysate)
A:HeLa S3
12% SDS PAGE
107-10691 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products