Anti-PKA C-alpha/PKA C-beta (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P17612
Gene Id	5566
Gene Symbols	PRKACA
Protein Name / Synonyms	PKA C-alpha/PKA C-beta (N-term)
Clonality	Polyclonal
Preparation	This PKA C-alpha/PKA C-beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-40 amino acids from the N-terminal region of human PKA C-alpha/PKA C-beta.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by the PRKACA gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase.

Specificity

Other Names

PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha

NCBI Accession #

NP_002721.1;NP_997401.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

40590

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-PKA/C alpha/beta Pab (Cat# 102-17067) is used in Western blot to detect PKA/C alpha in Jurkat cell lysate (lane 1) and mouse heart tissue lysate (lane 2).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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