Anti-PKC alpha Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	5578
Protein Name / Synonyms	PKC alpha
Concentration (lot specific)	0.69 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	PKC Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

AAG6 antibody, MGC129900 antibody, MGC129901 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, PRKCA antibody, PKC-A antibody, protein kinase C alpha type antibody, aging-associated gene 6 antibody, "protein kinase C, alpha antibody"

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

77 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 250 of PKC alpha (Uniprot ID# P17252)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Colon ca, using PKC alpha(107-10960) antibody at 1:500 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10960 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
107-10960 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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