Anti-PKC beta2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat, Non-Human Primate (rhesus monkey)
Host Species	Rabbit
Accession Number	P05771-2
Protein Name / Synonyms	PKC beta2 (C-term)
Clonality	Polyclonal
Preparation	This PKC beta2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 642-673 amino acids from the C-terminal region of human PKC beta2.
Concentration (lot specific)	1.930 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Angiogenesis, PKC Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC beta is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Isoform 1 uses an alternate exon at the 3' end compared to isoform 2, which includes a part of the coding region. The resulting isoform 1 has a distinct and shorter C-terminus, as compared to isoform 2.

Specificity

Other Names

PRKCB; PKCB; PRKCB1; Protein kinase C beta type

Other Accession #

P05127

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	The anti-PKC beta2 Pab (Cat# 102-17070) is used in Western blot to detect PKC beta2 in Jurkat cell lysate (lane 1) and mouse brain tissue lysate (lane 2).
	Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC beta2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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