Anti-PKC eta (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P24723
Gene Id	5583
Gene Symbols	PRKCH
Protein Name / Synonyms	PKC eta (N-term)
Clonality	Polyclonal
Preparation	This PKC eta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-239 amino acids from the N-terminal region of human PKC eta.
Concentration (lot specific)	1.160 mg/ml
Recommended Applications	Western Blotting
Research Area	PKC Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC eta is one of the PKC family members. This protein is most abundant in lung, less in heart and skin.

Specificity

Other Names

PRKCH; PKCL; PRKCL; Protein kinase C eta type; PKC-L; nPKC-eta

NCBI Accession #

NP_006246.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

77828

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-PKC eta N-term Pab (Cat# 102-17073) in NCI-H460 cell lysate. PKC eta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Western blot analysis of PRKCH (arrow) using PKC eta Antibody (N-term) (Cat# 102-17073).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCH gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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