Anti-PKC gamma (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P05129
Gene Id	5582
Gene Symbols	PRKCG
Protein Name / Synonyms	PKC gamma (C-term)
Clonality	Polyclonal
Preparation	This PKC gamma antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-336 amino acids from the C-terminal region of human PKC gamma.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	PKC Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).

Specificity

Predicted Reactivity

Monkey

Other Names

PRKCG; PKCG; Protein kinase C gamma type

NCBI Accession #

NP_002730.1

Other Accession #

Q4R4U2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

78448

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of anti-PKC gamma C-term Pab (Cat# 102-17074) in mouse brain lysate. PKC gamma (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC gamma antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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