Anti-PKC iota (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P41743
Gene Id	5584
Gene Symbols	PRKCI
Protein Name / Synonyms	PKC iota (N-term)
Clonality	Polyclonal
Preparation	This PKC iota antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-207 amino acids from the N-terminal region of human PKC iota.
Concentration (lot specific)	1.100 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	PKC Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PKC iota belongs to the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehydes-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis.

Specificity

Other Names

PRKCI; DXS1179E; Protein kinase C iota type; Atypical protein kinase C-lambda/iota; nPKC-iota

NCBI Accession #

NP_002731.4

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

68262

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of PRKCI (arrow) using rabbit polyclonal PKC iota Antibody (N-term) (Cat# 102-17075). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCI gene (Lane 2) (Origene Technologies).
	Western blot analysis of hPKC-iota-C181 (Cat# 102-17075) in Hela cell line lysates (35ug/lane).PKC (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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