Anti-PKC nu (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O94806
Gene Id	23683
Gene Symbols	PRKD3
Protein Name / Synonyms	PKC nu (C-term)
Clonality	Polyclonal
Preparation	This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 860-890 amino acids from the C-terminal region of human PKC nu.
Concentration (lot specific)	1.710 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	PKC Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.

Specificity

Predicted Reactivity

Mouse

Other Names

PRKD3; EPK2; PRKCN; Serine/threonine-protein kinase D3; Protein kinase C nu type; Protein kinase EPK2; nPKC-nu

NCBI Accession #

NP_005804.1

Other Accession #

Q8K1Y2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

100471

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-PKCnu C-term Pab (Cat# 102-17078) in NCI-H460 cell lysate. PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: GFP-PKD-transfected cells; lanes 3 and 7: GFP-PKD2-transfected cells; lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 N-term (102-17077) and C-term (102-17078) antibodies. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using 102-17077 and 102-17078 antibodies. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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