Anti-PKC theta (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q04759
Gene Id	5588
Gene Symbols	PRKCQ
Protein Name / Synonyms	PKC theta (C-term)
Clonality	Polyclonal
Preparation	This PKC theta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-348 amino acids from the C-terminal region of human PKC theta.
Concentration (lot specific)	1.140 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Inflammation, PKC Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PKC theta is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.

Specificity

Other Names

PRKCQ; PRKCT; Protein kinase C theta type; nPKC-theta

NCBI Accession #

NP_001229342.1;NP_006248.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

81865

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-PKC theta Pab (Cat# 102-17079) in placenta lysate. PKC theta (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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