Anti-PKC zeta (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q05513
Gene Id	5590
Gene Symbols	PRKCZ
Protein Name / Synonyms	PKC zeta (N-term)
Clonality	Polyclonal
Preparation	This PKC zeta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-197 amino acids from the N-terminal region of human PKC zeta.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Inflammation, PKC Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a constitutive kinase activity which is independent of calcium, and PKC activators, phosphatidylserine and diacylglycerol. Furthermore, it is insensitive to PKC inhibitors and cannot be activated by phorbol ester. The structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.

Specificity

Other Names

PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta

NCBI Accession #

NP_001028753.1;NP_001028754.1;NP_001229803.1;NP_002735.3

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

67660

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of anti-PKCzeta Pab (Cat# 102-17080) in placenta lysate. PKCzeta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PKC zeta antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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