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Anti-PKD2 Antibody
Catalog #: 107-10326
PKD2 antibody [C1C3], 0.99 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 25865 |
| Protein Name / Synonyms | PKD2 |
| Concentration (lot specific) | 0.99 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
PKD2 antibody, PRKD2 antibody, serine/threonine-protein kinase D2 antibody, protein kinase D2 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
97 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 529 and 862 of PKD2 (Uniprot ID# Q9BZL6)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
PKD2 antibody [C1C3] detects PKD2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PKD2 protein stained by PKD2 antibody [C1C3] (107-10326) diluted at 1:200.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PKD2 protein stained by PKD2 antibody [C1C3] (107-10326) diluted at 1:200.
Blue: Hoechst 33342 staining.
PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
PKD2 antibody [C1C3] (107-10326) dilution: 1:500.
Sample: Paraffin-embedded human breast carcinoma.
PKD2 antibody [C1C3] (107-10326) dilution: 1:500.
PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PKD2 antibody [C1C3] (107-10326) dilution: 1:500.
Sample: Paraffin-embedded mouse intestine.
PKD2 antibody [C1C3] (107-10326) dilution: 1:500.
Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10326 diluted at 1:1000
A: Molt-4
7.5% SDS PAGE
107-10326 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.