Anti-PKLR (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P30613
Gene Id	5313
Gene Symbols	PKLR
Protein Name / Synonyms	PKLR (N-term)
Clonality	Polyclonal
Preparation	This PKLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PKLR.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PKLR is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).

Specificity

Other Names

PKLR; PK1; PKL; Pyruvate kinase isozymes R/L; Pyruvate kinase 1; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase

NCBI Accession #

NP_000289.1;NP_870986.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

61830

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-PKLR Pab (Cat# 102-17095) is used in Western blot to detect PKLR in NIH/3T3 cell lysate.
	PKLR Antibody (S8) (Cat# 102-17095) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PKLR antibody detected the PKLR protein (arrow).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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