Anti-PLA2G4A (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P47712
Gene Id	5321
Gene Symbols	PLA2G4A
Protein Name / Synonyms	PLA2G4A (Center)
Clonality	Polyclonal
Preparation	This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-541 amino acids from the Central region of human PLA2G4A.
Concentration (lot specific)	0.410 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish, Chicken, Rabbit

Other Names

PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; Phospholipase A2 group IVA; Phospholipase A2; Phosphatidylcholine 2-acylhydrolase; Lysophospholipase

NCBI Accession #

NP_077734.1

Other Accession #

P50393; Q9TT38; P47713; P50392; P49147

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

85239

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of lysate from 293 cell line, using PLA2G4A Antibody (Center) (Cat# 102-17894). 102-17894 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
	Formalin-fixed and paraffin-embedded human lung carcinoma with PLA2G4A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of PLA2G4A Antibody (Center)(Cat# 102-17894) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	Flow cytometric analysis of NCI-H292 cells using PLA2G4A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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