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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P26678 |
| Gene Id | 5350 |
| Gene Symbols | PLN |
| Protein Name / Synonyms | PLB-T17 |
| Clonality | Polyclonal |
| Preparation | This PLB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human PLB. |
| Concentration (lot specific) | 0.340 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PLB is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.
Specificity
Other Names
PLN; PLB; Cardiac phospholamban
NCBI Accession #
NP_002658.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
6109
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of PLB-T17 (Cat# 102-18431) in Jurkat cell line lysates (35ug/lane). PLB (arrow) was detected using the purified Pab. |
 | PLB-T17 Antibody (Cat# 102-18431) IHC analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLB-T17 Antibody for immunohistochemistry. Clinical relevance has not been evaluated. |
 | PLB-T17 Antibody (Cat# 102-18431) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.