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Anti-PLCB1 Antibody
Catalog #: 144-01971
PLCB1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9NQ66 |
| Gene Id | 23236 |
| Gene Symbols | PLCB1 |
| Protein Name / Synonyms | PLCB1, EIEE12, PI-PLC, PLC-154, PLC-I, PLC-beta-1, PLC154, PLCB1A, PLCB1B, phospholipase C beta 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
133kDa/138kDa
Observed MW
145kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 917-1216 of human PLCB1 (NP_056007.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of NCI-H460 cells, using PLCB1 antibody (144-01971) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat heart using PLCB1 antibody (144-01971) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using PLCB1 antibody (144-01971) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PLCB1 antibody (144-01971) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PLCB1 antibody (144-01971). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.