Anti-PLCG1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P19174
Gene Id	5335
Gene Symbols	PLCG1
Protein Name / Synonyms	PLCG1, NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1, phospholipase C gamma 1
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Angiogenesis, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

148kDa

Observed MW

149kDa

Immunogen

A synthetic phosphorylated peptide around S1248 of human PLCG1 (NP_002651.2).

Modification

Phospho S1248

Storage

-20°C

Images

Western blot analysis of extracts of Jurkat cells, using Phospho-PLCG1-S1248 antibody (144-61540) at dilution or PLCG1 antibody (144-015704). Jurkat cells were treated by PMA/TPA (200 nM) at 37? for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - Phospho-PLCG1-S1248 pAb (144-61540)

Expiration:

12 months from the date of shipment when stored properly.

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