Anti-PLCG2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P16885
Gene Id	5336
Gene Symbols	PLCG2
Protein Name / Synonyms	PLCG2, APLAID, FCAS3, PLC-IV, PLC-gamma-2, phospholipase C gamma 2
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cancer, Inflammation, Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

147kDa

Observed MW

150kDa

Immunogen

A synthetic phosphorylated peptide around Y1217 of human PLCG2 (NP_002652.2).

Modification

Phospho Y1217

Storage

-20°C

Images

Western blot analysis of extracts of Raji cells, using Phospho-PLCG2-Y1217 antibody (144-61529) at 1:1000 dilution or PLCG2 antibody (144-02182). Raji cells were treated by Pervanadate (1 mM) at 37? for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - Phospho-PLCG2-Y1217 pAb (144-61529)

Expiration:

12 months from the date of shipment when stored properly.

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