Anti-Pleckstrin (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	5341
Protein Name / Synonyms	Pleckstrin, C-term
Concentration (lot specific)	0.61 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FLJ27168 antibody, P47 antibody, PLEK antibody, platelet 47 kDa protein antibody, pleckstrin antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

40 kDa

Immunogen

Synthetic peptide contain a sequence corresponding to a region within amino acids 287 and 300 of Pleckstrin

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Pleckstrin antibody [C2C3], C-term detects PLEK protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20? 100% MeOH for 5 min.
Green: PLEK protein stained by Pleckstrin antibody [C2C3], C-term (107-10609) diluted at 1:500.
Blue: Hoechst 33343 staining.

Sample(30 µg of whole cell lysate)
A:Raji
12% SDS PAGE
107-10609 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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