Rabbit Anti-Human PLOD1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q02809 |
| Gene Id | 5351 |
| Gene Symbols | PLOD1 |
| Protein Name / Synonyms | PLOD1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PLOD1. |
| Concentration (lot specific) | 0.320 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.
Specificity
Predicted Reactivity
Mouse
Other Names
PLOD1; LLH; PLOD; Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1; Lysyl hydroxylase 1
NCBI Accession #
NP_000293.2
Other Accession #
Q9R0E2; NP_000293.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
83550
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | PLOD1 Antibody (N-term) (Cat# 102-11678) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PLOD1 antibody detected the PLOD1 protein (arrow). |
 | PLOD1 Antibody (N-term) (Cat# 102-11678)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLOD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | PLOD1 Antibody (N-term) (Cat# 102-11678) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.