Anti-PNN (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9H307
Gene Id	5411
Gene Symbols	PNN
Protein Name / Synonyms	PNN (Center)
Clonality	Polyclonal
Preparation	This PNN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-239 amino acids from the Central region of human PNN.
Concentration (lot specific)	0.340 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.

Specificity

Predicted Reactivity

Mouse, Bovine

Other Names

PNN; DRS; MEMA; Pinin; 140 kDa nuclear and cell adhesion-related phosphoprotein; Desmosome-associated protein; Domain-rich serine protein; Melanoma metastasis clone A protein; Nuclear protein SDK3; SR-like protein

NCBI Accession #

NP_002678.2

Other Accession #

O35691; P79122; NP_002678.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

81614

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	PNN Antibody (Center) (Cat# 102-11145) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PNN antibody detected the PNN protein (arrow).
	PNN Antibody (Center) (Cat# 102-11145)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PNN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of PNN Antibody (Center)(Cat# 102-11145) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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