Anti-PNPase Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	87178
Protein Name / Synonyms	PNPase
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

DKFZp762K1914 antibody, OLD35 antibody, PNPASE antibody, old-35 antibody, PNPT1 antibody, 3'-5' RNA exonuclease OLD35 antibody, polynucleotide phosphorylase-like protein antibody, PNPase 1 antibody, PNPase old-35 antibody, "polyribonucleotide nucleotidyltransferase 1, mitochondrial antibody", polynucleotide phosphorylase 1 antibody, polyribonucleotide nucleotidyltransferase 1 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

86 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 783 of PNPase (Uniprot ID# Q8TCS8)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PNPase(107-11174) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2500.

PNPase antibody detects PNPase protein at mitochondria on human normal colon by immunohistochemical analysis.
Sample: Paraffin-embedded human normal colon.
PNPase antibody (107-11174) dilution: 1:250.

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
107-11174 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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