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Anti-POLR1A (C-term) Antibody
Catalog #: 102-14082
Rabbit Anti-Human POLR1A (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O95602 |
| Gene Id | 25885 |
| Gene Symbols | POLR1A |
| Protein Name / Synonyms | POLR1A (C-term) |
| Clonality | Polyclonal |
| Preparation | This POLR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1437-1465 amino acids from the C-terminal region of human POLR1A. |
| Concentration (lot specific) | 0.35 mg/ml |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).
Specificity
Other Names
POLR1A; DNA-directed RNA polymerase I subunit RPA1; A190; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; RNA polymerase I 194 kDa subunit
NCBI Accession #
NP_056240.2
Other Accession #
NP_056240.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
194811
Recommended Dilutions
WB: 1:1000
 | POLR1A Antibody (C-term) (Cat# 102-14082) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the POLR1A antibody detected the POLR1A protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.