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Anti-POLR2B Antibody
Catalog #: 144-05928
POLR2B Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P30876 |
| Gene Id | 5431 |
| Gene Symbols | POLR2B |
| Protein Name / Synonyms | POLR2B, POL2RB, RPB2, hRPB140, RNA polymerase II subunit B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
133kDa
Observed MW
134kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using POLR2B antibody (144-05928) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (144-05928) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (144-05928) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody (144-05928) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using POLR2B antibody (144-05928) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.