Anti-POLR3A Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	11128
Protein Name / Synonyms	POLR3A
Concentration (lot specific)	0.89 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

RPC1 antibody, RPC155 antibody, hRPC155 antibody, POLR3A antibody, DNA-directed RNA polymerase III subunit RPC1 antibody, RNA polymerase III subunit C160 antibody, DNA-directed RNA polymerase III subunit A antibody, RNA polymerase III subunit RPC155-D antibody, RNA polymerase III 155 kDa subunit antibody, DNA-directed RNA polymerase III largest subunit antibody, RNA polymerase III subunit C1 antibody, "polymerase (RNA) III (DNA directed) polypeptide A, 155kDa antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

156 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 182 and 482 of POLR3A (Uniprot ID# O14802)

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using POLR3A(107-10593) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.

Sample (30 ug of whole cell lysate)
A: HCT116
5% SDS PAGE
107-10593 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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