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Anti-PP1C gamma Antibody
Catalog #: 107-10538
PP1C gamma antibody [N1C2], 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 5501 |
| Protein Name / Synonyms | PP1C gamma |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
PP1gamma antibody, PPP1G antibody, PPP1CC antibody, protein phosphatase 1C catalytic subunit antibody, serine/threonine-protein phosphatase PP1-gamma catalytic subunit antibody, serine/threonine phosphatase 1 gamma antibody, "protein phosphatase 1, catalytic subunit, gamma isoform antibody", "protein phosphatase 1, catalytic subunit, gamma isozyme antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
37 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 2 and 227 of PP1C gamma (Uniprot ID# P36873)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (30 ug of whole cell lysate)
A: NIH-3T3 10% SDS PAGE
107-10538 diluted at 1:1000
A: NIH-3T3 10% SDS PAGE
107-10538 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP1 gamma(107-10538) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1CC(107-10538) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
B: Molt-4
10% SDS PAGE
107-10538 diluted at 1:1000
A: Hep G2
B: Molt-4
10% SDS PAGE
107-10538 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.