Anti-PPA1 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	5464
Protein Name / Synonyms	PPA1
Concentration (lot specific)	0.93 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

IOPPP antibody, MGC111556 antibody, PP antibody, PP1 antibody, SID6-8061 antibody, PPA1 antibody, pyrophosphate phospho-hydrolase antibody, cytosolic inorganic pyrophosphatase antibody, inorganic pyrophosphatase 1 antibody, inorganic diphosphatase antibody, pyrophosphatase 1 antibody, inorganic pyrophosphatase antibody, diphosphate phosphohydrolase antibody, PPase antibody, pyrophosphatase (inorganic) 1 antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

33 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 289 of PPA1 (Uniprot ID# Q15181)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

PPA1 antibody detects PPA1 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
12 % SDS-PAGE
PPA1 antibody (107-10946) dilution: 1:1000

PPA1 antibody detects PPA1 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
PPA1 antibody (107-10946) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPA1(107-10946) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PPA1(107-10946) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2
D: Molt-4
12% SDS PAGE
107-10946 diluted at 1:10000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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