Rabbit Anti-PPIA (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P62937 |
| Gene Id | 5478 |
| Gene Symbols | PPIA |
| Protein Name / Synonyms | PPIA (N-term) |
| Clonality | Polyclonal |
| Preparation | This PPIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-37 amino acids from the N-terminal region of human PPIA. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PPIA is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions.
Specificity
Predicted Reactivity
Rat, Bovine, Hamster, Pig, Rabbit
Other Names
PPIA; CYPA; Peptidyl-prolyl cis-trans isomerase A; Cyclophilin A; Cyclosporin A-binding protein; Rotamase A
NCBI Accession #
NP_066953.1
Other Accession #
P10111; Q9TTC6; P62936; P17742; P14851; P62935
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
18012
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of PPIA Antibody (N-term) (Cat# 102-15244) in T47D cell line(lane 1) and mouse brain(lane 2),bladder(lane 3) tissue lysates (35ug/lane). PPIA (arrow) was detected using the purified Pab.(2ug/ml) |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with PPIA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of widr cells using PPIA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.