RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-PPM1K Antibody

Catalog #: 107-10527

PPM1K antibody [N1C3], 1 mg/ml (100 ul)

Product Description

Specifications

Size100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Gene Id
152926
Protein Name / Synonyms
PPM1K
Concentration (lot specific)
1 mg/ml
Recommended ApplicationsIHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping TypeBlue ice
Storage-20°C

Description

Synonyms

DKFZp667B084 antibody, DKFZp761G058 antibody, PP2Ckappa antibody, PP2Cm antibody, PTMP antibody, UG0882E07 antibody, hPTMP antibody, PPM1K antibody, protein phosphatase 2C kappa antibody, PP2C-type mitochondrial phosphoprotein phosphatase antibody, "protein phosphatase 1K, mitochondrial antibody", PP2C domain-containing protein phosphatase 1K antibody, PP2C-kappa antibody, protein phosphatase 2C isoform kappa antibody, PP2C-like mitochondrial protein antibody, PP2C-like protein antibody, protein phosphatase 1K (PP2C domain containing) antibody, PP2C type mitochondrial phosphatase antibody, "protein phosphatase, Mg2+/Mn2+ dependent, 1K antibody"

Specificity

Species Reactivity
Human

Format

Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
41 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 364 of PPM1K (Uniprot ID# Q8N3J5)

Applications

Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded endo mitral OVCA, using PPM1K(107-10527) antibody at 1:100 dilution.
Western Blot analysis of PPM1K expression in transfected 293T cell line by PPM1K polyclonal antibody.
A: Non-transfected lysate.
B: PPM1K transfected lysate.
12% SDS PAGE
107-10527 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.