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Anti-PPP1CA Antibody
Catalog #: 144-61520
Phospho-PPP1CA-T320 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P62136 |
| Gene Id | 5499 |
| Gene Symbols | PPP1CA |
| Protein Name / Synonyms | PPP1CA, PP-1A, PP1A, PP1alpha, PPP1A, protein phosphatase 1 catalytic subunit alpha |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
32kDa/37kDa/38kDa
Observed MW
38kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding T320 of human PPP1CA
Modification
Phospho T320
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-PPP1CA-T320 antibody (144-61520) at 1:2000 dilution or PPP1CA antibody (144-12468). NIH/3T3 cells were treated by Calyculin A (100 nM) at 37? for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 30s.
Expiration:
12 months from the date of shipment when stored properly.