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Anti-PPP1R12A Antibody
Catalog #: 144-61513
Phospho-PPP1R12A-S507 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O14974 |
| Gene Id | 4659 |
| Gene Symbols | PPP1R12A |
| Protein Name / Synonyms | PPP1R12A, M130, MBS, MYPT1, protein phosphatase 1 regulatory subunit 12A |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
105kDa/109kDa/111kDa/115kDa
Observed MW
135kDa
Immunogen
A synthetic phosphorylated peptide around S507 of human PPP1R12A (NP_001137357.1).
Modification
Phospho S507
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-PPP1R12A-S507 antibody (144-61513) at 1:2000 dilution or PPP1R12A antibody (144-00587). HeLa cells were treated by Calyculin A (100 nM) at 37? for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37? for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.