RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-PPP3CB Antibody

Catalog #: 107-10951

PPP3CB antibody [N1N3], 1 mg/ml (100 ul)

Product Description

Specifications

Size100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Gene Id
5532
Protein Name / Synonyms
PPP3CB
Concentration (lot specific)
1 mg/ml
Recommended ApplicationsIHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping TypeBlue ice
Storage-20°C

Description

Synonyms

CALNA2 antibody, CALNB antibody, CNA2 antibody, PP2Bbeta antibody, PPP3CB antibody, protein phosphatase from PCR fragment H32 antibody, calmodulin-dependent calcineurin A subunit beta isoform antibody, "protein phosphatase 2B, catalytic subunit, beta isoform antibody", calcineurin A beta antibody, serine/threonine-protein phosphatase 2B catalytic subunit beta isoform antibody, calcineurin A2 antibody, CAM-PRP catalytic subunit antibody, "protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform antibody", "protein phosphatase 3, catalytic subunit, beta isozyme antibody"

Specificity

Species Reactivity
Human, Mouse

Format

Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
59 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 228 of PPP3CB (Uniprot ID# P16298)

Applications

Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using PPP3CB(107-10951) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10951 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10951 diluted at 1:5000

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.