Anti-PPT1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P50897
Gene Id	5538
Gene Symbols	PPT1
Protein Name / Synonyms	PPT1 (C-term)
Clonality	Polyclonal
Preparation	This PPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-300 amino acids from the C-terminal region of human PPT1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.

Specificity

Predicted Reactivity

Monkey

Other Names

PPT1; PPT; Palmitoyl-protein thioesterase 1; Palmitoyl-protein hydrolase 1

NCBI Accession #

NP_000301.1;NP_001136076.1

Other Accession #

Q8HXW6

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

34193

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of PPT1 Antibody (C-term) (Cat# 102-15056) in 293 cell line lysates (35ug/lane). PPT1 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PPT1 antibody (C-term) (Cat# 102-15056), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Western blot analysis of PPT1 (arrow) using rabbit polyclonal PPT1 Antibody (A284) (Cat# 102-15056). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPT1 gene.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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