Mouse Anti-PRDM2 (RIZ1) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Accession Number | Q13029 |
| Gene Id | 7799 |
| Gene Symbols | PRDM2 |
| Protein Name / Synonyms | PRDM2 (RIZ1) |
| Clonality | Monoclonal |
| Preparation | Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. This antibody is specific for RIZ1, it does not recognize the isoform beta (RIZ2). |
| Concentration (lot specific) | 1.250 mg/ml |
| Recommended Applications | Western Blotting, IP, CHIP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene.
Specificity
Other Names
PRDM2; KMT8; RIZ; PR domain zinc finger protein 2; GATA-3-binding protein G3B; Lysine N-methyltransferase 8; MTB-ZF; MTE-binding protein; PR domain-containing protein 2; Retinoblastoma protein-interacting zinc finger protein; Zinc finger protein RIZ
NCBI Accession #
NP_001007258.1;NP_001129082.1;NP_036363.2;NP_056950.2
Format
Type
Purified Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
188915
Recommended Dilutions
WB: 1:1000; CHIP: 1:100
 | Western blot analysis of anti-PRDM2 Mab (Cat# 102-10037) in lysate from PRDM2 transgenic lysate.Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence. |
 | ChIP analysis of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with antibody against RIZ1 (102-10037). DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter. Adapted from Fig 6 in Carling et al., 2004 (see citation 2). |
 | ChIP analysis of estrogen target genes.(B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with antibody to RIZ1 (102-10037) and control immunoglobulin G as indicated. Adapted from Fig 6 in Carling et al., 2004 (see citation 2). |
 | ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts by using anti-RIZ1 antibody (102-10037). Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in Carling et al., 2004 (see citation 2). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.