Anti-PRDX2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P32119
Gene Id	7001
Gene Symbols	PRDX2
Protein Name / Synonyms	PRDX2 (C-term)
Clonality	Polyclonal
Preparation	This PRDX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the C-terminal region of human PRDX2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PRDX2 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms.

Specificity

Predicted Reactivity

Rat, Bovine, Hamster, Monkey

Other Names

PRDX2; NKEFB; TDPX1; Peroxiredoxin-2; Natural killer cell-enhancing factor B; PRP; Thiol-specific antioxidant protein; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1

NCBI Accession #

NP_005800.3

Other Accession #

P35704; Q61171; Q2PFZ3; Q8K3U7; Q9BGI3

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

21892

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:50-100

	Fluorescent confocal image of Hela cell stained with PRDX2 Antibody (C-term)(Cat# 102-15232).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRDX2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).PRDX2 immunoreactivity is localized to Cytoplasm significantly.
	Western blot analysis of PRDX2 Antibody (C-term) (Cat# 102-15232) in MDA-MB231,293 cell line and mouse brain tissue lysates (35ug/lane).PRDX2 (arrow) was detected using the purified Pab.(2ug/ml)
	Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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