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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P51888 |
| Gene Id | 5549 |
| Gene Symbols | PRELP |
| Protein Name / Synonyms | PRELP (C-term) |
| Clonality | Polyclonal |
| Preparation | This PRELP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the C-terminal region of human PRELP. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PRELP is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage.
Specificity
Other Names
PRELP; SLRR2A; Prolargin; Proline-arginine-rich end leucine-rich repeat protein
NCBI Accession #
NP_002716.1;NP_958505.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
43810
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of PRELP antibody (C-term) (Cat# 102-16878) in Y79 cell line lysates (35ug/lane). PRELP (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma with PRELP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of widr cells using PRELP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.