Anti-PRKAA2 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Accession Number	P54646
Gene Id	5563
Gene Symbols	PRKAA2
Protein Name / Synonyms	PRKAA2
Clonality	Monoclonal
Preparation	This PRKAA2 monoclonal antibody is generated from mouse immunized with PRKAA2 recombinant protein.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Specificity

Other Names

PRKAA2; AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase

NCBI Accession #

NP_006243.2

Other Accession #

NP_006243.2

Format

Type

Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

62320

Recommended Dilutions

IF: 1:10-50; WB: 1:100-500

Fluorescent image of Hela cell stained with PRKAA2 Antibody(Cat# 102-10116/SG110126AA).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRKAA2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).PRKAA2 immunoreactivity is localized to Cytoplasm significantly.

PRKAA2 antibody (Cat# 102-10116) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PRKAA2 antibody detected the PRKAA2 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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