Anti-PRKAA2 (Thr172) (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P54646
Gene Id	5563
Gene Symbols	PRKAA2
Protein Name / Synonyms	PRKAA2 (Thr172) (Center)
Clonality	Polyclonal
Preparation	This PRKAA2 (Thr172) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the Central region of human PRKAA2 (Thr172).
Concentration (lot specific)	0.450 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Specificity

Predicted Reactivity

Mouse, Rat, Pig

Other Names

PRKAA2; AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase

NCBI Accession #

NP_006243.2

Other Accession #

Q09137; Q28948; Q8BRK8; P54645; Q09136; Q5EG47; Q13131; NP_006243.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

62320

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	PRKAA2 (Thr172) Antibody (Cat# 102-10824) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PRKAA2 antibody detected the PRKAA2 protein (arrow).
	PRKAA2 (Thr172) antibody (Center) (Cat# 102-10824) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the PRKAA2 (Thr172) antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	PRKAA2 (Thr172) Antibody (Center) (Cat# 102-10824) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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