Rabbit Anti-PRKCA (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P17252 |
| Gene Id | 5578 |
| Gene Symbols | PRKCA |
| Protein Name / Synonyms | PRKCA (N-term) |
| Clonality | Polyclonal |
| Preparation | This PRKCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-103 amino acids from the N-terminal region of human PRKCA. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PRKCA is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. It may play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Specificity
Predicted Reactivity
Rat, Bovine, Rabbit
Other Names
PRKCA; PKCA; PRKACA; Protein kinase C alpha type
NCBI Accession #
NP_002728.1
Other Accession #
P05696; P10102; P20444; P04409
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
76750
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of PRKCA Antibody (N-term) (Cat# 102-17925) in mouse NIH-3T3 cell line lysates (35ug/lane). PRKCA (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRKCA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | PRKCA Antibody (N-term) (Cat# 102-17925) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of PRKCA Antibody (N-term)(Cat# 102-17925) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.