Phospho-PKC (pan) (?II Ser660) pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P17252|Q04759|Q05655|P05771|Q02156|P24723 |
| Gene Id | 5578|5588|5580|5579|5581|5583 |
| Gene Symbols | PRKCA, PRKCB, PRKCD, PRKCQ, PRKCE, PRKCH |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | PKC Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
59kDa/67kDa/74- 83kDa
Observed MW
95kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding Ser660 of human PKC (pan) ?II
Modification
Phospho-PKC (pan) (?II Ser660)
Storage
-20°C
Images
Western blot analysis of extracts of 293T cells, using Phospho-PKC (pan) (?II Ser660) antibody (144-61499) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.