Anti-PRKD1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q15139
Gene Id	5587
Gene Symbols	PRKD1
Protein Name / Synonyms	PRKD1, CHDED, PKC-MU, PKCM, PKD, PRKCM, protein kinase D1
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Angiogenesis, Cardiovascular Disease, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

101kDa

Observed MW

102kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S744/748 of human PKD/PKCmu

Modification

Phospho S744/748

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using Phospho-PKD/PKCmu-S744/748 antibody (144-61503) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight or treated by PDGF (100ng/ml) for 30 minutes after serum-starvation overnight.C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight or treated by PDGF (50 ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - Phospho-PKD/PKCmu-S744/748 antibody (144-61503)

Expiration:

12 months from the date of shipment when stored properly.

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