Anti-PRMT2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P55345
Gene Id	3275
Gene Symbols	PRMT2
Protein Name / Synonyms	PRMT2 (N-term)
Clonality	Polyclonal
Preparation	This PRMT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-53 amino acids from the N-terminal region of human PRMT2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	IHC-P, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

Specificity

Other Names

PRMT2; HMT1; HRMT1L1; Protein arginine N-methyltransferase 2; Histone-arginine N-methyltransferase PRMT2

NCBI Accession #

NP_001229793.1;NP_001229794.1;NP_001526.2;NP_996845.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

49042

Recommended Dilutions

IHC: 1:50-100; WB: 1:1000

	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	The anti-PRMT2 Pab (Cat# 102-10532) is used in Western blot to detect PRMT2 in HL60 cell lysate.
	Western blot analysis of PRMT2 (arrow) using rabbit polyclonal PRMT2 Antibody (A37) (Cat# 102-10532). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRMT2 gene.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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