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Anti-PRMT4 (Center) Antibody

Catalog #: 102-11041

Rabbit Anti-Human PRMT4(Center) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q86X55
Gene Id
10498
Gene Symbols
CARM1
Protein Name / Synonyms
PRMT4(Center)
ClonalityPolyclonal
PreparationThis PRMT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-377 amino acids from the Central region of human PRMT4.
Concentration (lot specific)
0.5 mg/ml
Recommended ApplicationsWestern Blotting, IHC-P, FC
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).

Specificity

Predicted Reactivity
Mouse, Rat
Other Names
CARM1; PRMT4; Histone-arginine methyltransferase CARM1; Coactivator-associated arginine methyltransferase 1; Protein arginine N-methyltransferase 4
NCBI Accession #
NP_954592.1
Other Accession #
Q4AE70; Q9WVG6; NP_954592.1

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
65854
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
PRMT4 Antibody(Center) (Cat# 102-11041) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PRMT4 antibody detected the PRMT4 protein (arrow).
PRMT4 Antibody (Center) (Cat# 102-11041)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRMT4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
PRMT4 Antibody(Center) (Cat# 102-11041) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.