Anti-PRMT5 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O14744
Gene Id	10419
Gene Symbols	PRMT5
Protein Name / Synonyms	PRMT5 (C-term)
Clonality	Polyclonal
Preparation	This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 591-621 amino acids from the C-terminal region of human PRMT5.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

Specificity

Predicted Reactivity

Mouse, Bovine, Monkey

Other Names

PRMT5; HRMT1L5; IBP72; JBP1; SKB1; Protein arginine N-methyltransferase 5; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog; Protein arginine N-methyltransferase 5, N-terminally processed

NCBI Accession #

NP_001034708.1;NP_006100.2

Other Accession #

Q8CIG8; Q4R5M3; A7YW45

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

72684

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-PRMT5 Pab (Cat# 102-10546) is used in Western blot to detect PRMT5 in HL60 cell lysate.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products