Rabbit Anti-Human Pro-BNP Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NP_002512 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was a recombinant proteinderived from Human pro-BNP. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 20, 000(at least detecting 50ng/ml)), Western Blotting (recommended work dilution= 1:1, 000-5, 000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein proBNP, lane 2. T98G cell lysate. The membrane blot was probed with anti-proBNP primary antibody (0.25?g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular proBNP from human and mouse cells (35 kDa). |
| Antigen Catalog Number | 230-00009 |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. |
| Research Area | Neuroscience, Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
The natriuretic peptides B proprotein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
Specificity
The rabbit anti-human natriuretic peptide B proprotein antibody detects specifically target derived from human recombinant protein as well as T98G cell lysates. Cross reactivity with mouse and rat pro-BNP has not yet been tested.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
Related Products
Mouse Anti-proBNP, N-terminal (Cat# MD-17-0034 )Human proBNP, N-terminal Recombinant protein. (Cat# 230-00009)
References
- Dan L. Crimmins and Jeffrey L.-H. Kao, (2008) A glycosylated form of the human cardiac hormone pro B-type natriuretic peptide is an intrinsically unstructured monomeric protein. Arch Biochem Biophys.1;475(1): 3641.
Expiration:
12 months from the date of shipment when stored properly.
