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Anti-Pro-BNP Antibody
Catalog #: 130-10070
Mouse Anti-Human Pro-BNP Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | The Mouse anti-human proBNP antibody specifically detects target derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity. |
| Accession Number | P16860 |
| Gene Id | 4879 |
| Gene Symbols | NPPB |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein derived from proBNP. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | Direct ELISA: antibody detection limit dilution 1:2, 000. Recommended for use at 100 ng/ml. Optimal dilutions should be determined by each laboratory for each application. |
| Antigen Catalog Number | 230-00009 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Angiogenesis, Neuroscience, Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Brain natriuretic peptide (BNP) is a polypeptide of 134-amino acids comprised of a 26-amino acid signal peptide (Met1-Ser26), a 76-amino acid N-terminal fragment (NT-proBNP) (His27-Arg102), and a 32-amino acid mature polypeptide of brain natriuretic peptide 32 (BNP-32)(Ser103-His134). BNP-32 is secreted by the ventricles of the heart in response to excessive stretching of heart muscle cells (cardiomyocytes). BNP-32 is co-secreted along with NT-proBNP which is biologically inactive. Both BNP-32 and NT-proBNP markers are used for screening, diagnosis of acute congestive heart failure (CHF). The plasma concentrations of both BNP and NTproBNP are also typically increased in patients with asymptomatic/symptomatic left ventricular dysfunction.
Typical Data
Storage/Stability
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Expiration
12 months from the date of shipment when stored properly.
References
- Bhalla V, et al. (2004). B-type natriuretic peptide: the level and the drug--partners in the diagnosis of congestive heart failure. Congest Heart Fail 10 (1 Suppl 1): 327.
- Atisha D, et al. (2004). A prospective study in search of an optimal B-natriuretic peptide level to screen patients for cardiac dysfunction. Am. Heart J. 148 (3): 51823.
- Maisel A, et al. (2002). Rapid measurement of B-type natriuretic peptide in the emergency diagnosis of heart failure. N Engl J Med 347 (3): 1617.