3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Pro-BNP Antibody, Biotinylated
Catalog #: 130-10015B-50
Rabbit anti-Pro-BNP Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | NP_002512 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was a recombinant proteinderived from Human pro-BNP. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 20, 000(at least detecting 50 µg/ml)), Western Blotting (recommended work dilution= 1:1, 000-5, 000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein proBNP, lane 2. T98G cell lysate. The membrane blot was probed with anti-proBNP primary antibody (0.25 µg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular proBNP from human and mouse cells (35 kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Neuroscience, Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The natriuretic peptides B proprotein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-human natriuretic peptide B proprotein antibody detects specifically target derived from human recombinant protein as well as T98G cell lysates. Cross reactivity with mouse and rat pro-BNP has not yet been tested.
Related Products
Mouse Anti-proBNP, N-terminal (Cat# MD-17-0034 )Human proBNP, N-terminal Recombinant protein. (Cat# 230-00009)
References
- Dan L. Crimmins and Jeffrey L.-H. Kao, (2008) A glycosylated form of the human cardiac hormone pro B-type natriuretic peptide is an intrinsically unstructured monomeric protein. Arch Biochem Biophys.1;475(1): 3641.
Expiration:
12 months from the date of shipment when stored properly.