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Anti-pro-BNP (NPPB) Antibody
Catalog #: 130-10043
Rabbit Anti-Human pro-BNP (NPPB) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P16860 |
| Gene Id | 4879 |
| Gene Symbols | NPPB |
| Clonality | Polyclonal |
| Immunogen | Immunogen was human expressed proBNP long peptide(76 Amino acid of N-terminal fragment). This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography |
| Recommended Applications | ELISA (recommended work dilution= 1: 16, 000(at least detecting 62.5 ng/ml)) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Angiogenesis, Neuroscience, Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Brain natriuretic peptide (BNP) is a polypeptide of 134-amino acids comprised of a 26-amino acid signal peptide (Met1-Ser26), a 76-amino acid N-terminal fragment (NT-proBNP) (His27-Arg102), and a 32-amino acid mature polypeptide of brain natriuretic peptide 32 (BNP-32)(Ser103-His134). BNP-32 is secreted by the ventricles of the heart in response to excessive stretching of heart muscle cells (cardiomyocytes). BNP-32 is cosecreted along with NT-proBNP which is biologically inactive. Both BNP-32 and NT-proBNP markers are used for screening, diagnosis of acute congestive heart failure (CHF). The plasma concentrations of both BNP and NTproBNP are also typically increased in patients with asymptomatic/symptomatic left ventricular dysfunction.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat proBNP has not yet been tested.
Storage
Keep it at 4°C if intended for use within a month. Store at -20°C if over months. Minimize freezing and thawing when use.
Related Products
Rabbit Anti-Human pro-Brain Natriuretic Peptide? Cat# 130-10015RayBio® Brain Natriuretic Peptide (BNP) EIA Kit, Cat# EIA-BNP-1 Recombinant Human pro-Brain Natriuretic Peptide, 230-00009-1
References
- Dan L.et al., (2008) A glycosylated form of the human cardiac hormone pro B-type natriuretic peptide is an intrinsically unstructured monomeric protein. Arch Biochem Biophys.475(1): 3641.
Expiration:
12 months from the date of shipment when stored properly.