3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-pro-BNP (NPPB) Antibody, Biotinylated
Catalog #: 130-10043B-50
Rabbit anti-pro-BNP (NPPB) Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P16860 |
| Gene Id | 4879 |
| Gene Symbols | NPPB |
| Clonality | Polyclonal |
| Immunogen | Immunogen was human expressed proBNP long peptide(76 Amino acid of N-terminal fragment). This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 16, 000(at least detecting 62.5 µg/ml)) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Angiogenesis, Neuroscience, Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Brain natriuretic peptide (BNP) is a polypeptide of 134-amino acids comprised of a 26-amino acid signal peptide (Met1-Ser26), a 76-amino acid N-terminal fragment (NT-proBNP) (His27-Arg102), and a 32-amino acid mature polypeptide of brain natriuretic peptide 32 (BNP-32)(Ser103-His134). BNP-32 is secreted by the ventricles of the heart in response to excessive stretching of heart muscle cells (cardiomyocytes). BNP-32 is cosecreted along with NT-proBNP which is biologically inactive. Both BNP-32 and NT-proBNP markers are used for screening, diagnosis of acute congestive heart failure (CHF). The plasma concentrations of both BNP and NTproBNP are also typically increased in patients with asymptomatic/symptomatic left ventricular dysfunction.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat proBNP has not yet been tested.
Related Products
Rabbit Anti-Human pro-Brain Natriuretic Peptide? Cat# 130-10015RayBio® Brain Natriuretic Peptide (BNP) EIA Kit, Cat# EIA-BNP-1 Recombinant Human pro-Brain Natriuretic Peptide, 230-00009-1
References
- Dan L.et al., (2008) A glycosylated form of the human cardiac hormone pro B-type natriuretic peptide is an intrinsically unstructured monomeric protein. Arch Biochem Biophys.475(1): 3641.
Expiration:
12 months from the date of shipment when stored properly.