Anti-Proteasome 20S alpha 6 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	5687
Protein Name / Synonyms	Proteasome 20S alpha 6
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

IOTA antibody, MGC22756 antibody, MGC2333 antibody, MGC23846 antibody, PROS27 antibody, p27K antibody, PSMA6 antibody, 27 kDa prosomal protein antibody, multicatalytic endopeptidase complex iota chain antibody, macropain iota chain antibody, prosomal P27K protein antibody, proteasome subunit alpha type-6 antibody, PROS-27 antibody, macropain subunit iota antibody, proteasome iota chain antibody, proteasome subunit iota antibody, proteasome (prosome, macropain) subunit, alpha type, 6 antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

27 kDa

Immunogen

Recombinant fragment contain a sequence corresponding to a region within amino acids 57 and 274 of Proteasome 20S alpha 6

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
12 % SDS-PAGE
Proteasome 20S alpha 6 antibody (107-11073) dilution: 1:1000

Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis.
A. 50 µg rat liver lysate/extract
12 % SDS-PAGE
Proteasome 20S alpha 6 antibody (107-11073) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed A431, using 20S Proteasome alpha6 (107-11073) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using 20S Proteasome alpha6 (107-11073) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: H1299
D: Hela
12% SDS PAGE
107-11073 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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