Anti-Proteasome 20S alpha 7 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	5688
Protein Name / Synonyms	Proteasome 20S alpha 7
Concentration (lot specific)	0.86 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

C6 antibody, HSPC antibody, MGC3755 antibody, RC6-1 antibody, XAPC7 antibody, PSMA7 antibody, proteasome subunit alpha type-7 antibody, proteasome subunit alpha 4 antibody, proteasome subunit XAPC7 antibody, proteasome subunit RC6-1 antibody, "proteasome (prosome, macropain) subunit, alpha type, 7 antibody"

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

28 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 205 of Proteasome 20S alpha 7 (Uniprot ID# O14818)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
107-11074 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using proteasome alpha 7(107-11074) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
12% SDS PAGE
107-11074 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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